Fluoroquinolone drugs (QNS) are a very important broad-spectrum antibiotic that has rapidly developed in the past 20 years. They can inhibit bacterial DNA helicase, have a wide antibacterial spectrum, high efficiency, low toxicity, and strong tissue penetration. It has become one of the most important anti infective drugs in veterinary clinics and aquaculture, widely used for treatment, prevention, and growth promotion. Its drug resistance and potential carcinogenicity have attracted widespread attention. Announcement No. 235 of the Ministry of Agriculture stipulates that the total amount of fluoroquinolone drugs in animal tissues shall not exceed 100 μ On September 1, 2015, the Ministry of Agriculture issued Announcement No. 2292, which prohibits the use of four veterinary drugs: lomefloxacin, pefloxacin, norfloxacin, and ofloxacin. This reagent kit only takes 45 minutes to operate.