
The protein G affinity column utilizes the specific adsorption between protein G and immunoglobulin to purify and separate immunoglobulins in the sample, and then achieves quantitative detection of immunoglobulins through liquid chromatography.
IgG is an important immune factor, and clinical studies have shown that IgG in milk plays a potential and positive immune role in prevention and treatment. In the development of colostrum products, measuring the content of IgG can indicate the quality of the product.
According to the principle of high-performance affinity chromatography, immunoglobulin IgG is linked to ligands in phosphate buffer and eluted with glycine hydrochloride at pH 2.5.