Fuma Toxin Immunoaffinity Column
The immunoaffinity column can selectively adsorb Fumaroxins (FB1, FB2, FB3) in the sample solution, thereby providing a highly targeted purification effect on the sample. After column purification, the sample solution can be directly used for LC-MS/MS analysis or derivatized and analyzed by HPLC.
The use of affinity columns in combination with LC-MS/MS and HPLC can achieve rapid detection, improve signal-to-noise ratio, and enhance the accuracy of detection methods.
Product overview
Fumonisin is a metabolite produced by the genus Fusarium, with acute and chronic toxicity, as well as species and organ specificity. Studies have found that high concentrations of Fumonisin can cause species specific acute toxic symptoms in various domestic and experimental animals, such as equine leukomalacia syndrome, pulmonary edema in pigs, and liver and kidney lesions in sheep. It has also been found that Fumonisin may be related to human esophageal cancer and liver cancer.
Product principle
The basis of the determination is antigen antibody reaction, where the antibody is connected to the column. After extraction and filtration, the sample slowly passes through the Fumarotoxin immunoaffinity column, where the endotoxin binds to the antibody. Afterwards, the immunoaffinity column is washed to remove any other unrelated substances that have not been bound. Wash off the Fumarotoxin with eluent, then inject it into the analytical instrument or inject it into the analytical instrument after derivatization for detection.
