The rapid detection strip of chloramphenicol applies the principle of competitive inhibition immunochromatography. During the flow process, chloramphenicol in the sample binds to a colloidal gold labeled specific chloramphenicol monoclonal antibody, inhibiting the binding of the antibody to the chloramphenicol BSA conjugate on the NC membrane detection line, resulting in a change in the color of the detection line. When the sample does not contain chloramphenicol or the concentration is below the detection limit, When the concentration of chloramphenicol in the sample is equal to or higher than the detection limit, the T-line color is stronger than the C-line color or there is no significant difference from the C-line color, The color development of T-line is significantly weaker than that of C-line or T-line does not show color, and regardless of whether the sample contains chloramphenicol quality control line, C-line will show color to indicate effective detection.