T-2 toxin test strip
The rapid quantitative test strip for T-2 toxin uses colloidal gold competition method and specific antigen antibody reaction for rapid qualitative detection of T-2 toxin in grain, feed raw materials and some finished feed products. It is suitable for on-site rapid detection by various enterprises, testing institutions and supervisory departments.
Detection steps
1. Before conducting the test, read the user manual completely, remove the reagent bucket from the original packaging, open it, take out the required amount of microporous reagents and test strips, and mark them properly; After removing the microporous reagent, please immediately cover the reagent barrel lid.
2. Before use, restore the test strip, microporous reagent, and sample to room temperature (20-25 ℃); Place the required microporous reagent in an empty microporous plate rack; The number of samples tested at once should not exceed 8; If it exceeds the limit, it can be tested in batches according to groups of 8.
3. Take the test solution and use a micropipette to transfer 150 μ L into the micropore. Slowly aspirate until the test sample and micropore reagent are thoroughly mixed about 5-6 times, and start counting with a timer.
After incubating at room temperature (20-25 ℃) for 4 minutes, immerse the labeled test strip with the handle end with the logo facing up and the other end facing down into the solution, and then start timing.
5. After reacting at room temperature (20-25 ℃) for 5 minutes, remove the sample pad at the bottom of the test strip and read the results within 1 minute according to the schematic diagram. Other times of reading are invalid.
Matters needing attention
1. Before use, restore the test strip and the sample to room temperature.
2. Please use the test strip within its validity period.
3. When opening the microporous reagent, gently remove the sealing film and do not use force to quickly remove it to prevent powder from flying out.
4. Please use up the microporous reagent as soon as possible after opening, otherwise it will become ineffective and affect the detection effect.
5. Do not touch the white film surface in the center of the test strip.
6. Avoid direct sunlight and direct fan blowing during testing.
7. Do not use tap water, purified water, and distilled water as negative controls.
8. For the extraction of the supernatant of corn germ meal, sprayed corn husk, corn protein powder, and some finished feed, if it is found to be acidic or alkaline after extraction, it is best to use 2.0 mol/L NaOH solution or 2.0 mol/L HCl solution to adjust the pH of the supernatant to 6.0-8.0 before diluting and testing.
9. Due to the complex formula of finished feed, it may contain substances that interfere with detection; If you have any questions during the testing, please contact the supplier promptly.
10. This method is a screening method. In case of positive or suspected positive samples, please confirm them using the confirmatory method.
11. If you encounter any problems during the experiment, please contact the supplier.
