{"id":2136,"date":"2023-04-19T05:00:49","date_gmt":"2023-04-19T05:00:49","guid":{"rendered":"https:\/\/www.vnyabiotech.com\/?p=2136"},"modified":"2023-04-26T03:20:51","modified_gmt":"2023-04-26T03:20:51","slug":"vitamin-b12-elisa-kit","status":"publish","type":"post","link":"https:\/\/www.vnyabiotech.com\/ja\/vitamin-b12-elisa-kit\/","title":{"rendered":"\u30d3\u30bf\u30df\u30f3B12 ELISA\u30ad\u30c3\u30c8"},"content":{"rendered":"
\n\t\t\t\t\t\t
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<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\"ELISA<\/figure><\/div>\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\u30d3\u30bf\u30df\u30f3B12 ELISA\u30ad\u30c3\u30c8<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u30d3\u30bf\u30df\u30f3 B12 (\u30b7\u30a2\u30ce\u30b3\u30d0\u30e9\u30df\u30f3) ELISA \u30ad\u30c3\u30c8\u306f\u3001\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d44\u7e54\u30db\u30e2\u30b8\u30cd\u30fc\u30c8\u304a\u3088\u3073\u305d\u306e\u4ed6\u306e\u4f53\u6db2\u4e2d\u306e\u30d3\u30bf\u30df\u30f3 B12 (\u30b7\u30a2\u30ce\u30b3\u30d0\u30e9\u30df\u30f3) \u6fc3\u5ea6\u3092 in vitro \u3067\u5b9a\u91cf\u6e2c\u5b9a\u3059\u308b\u305f\u3081\u306e ELISA \u30ad\u30c3\u30c8\u3067\u3059\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\n\t\t\t\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u4ed5\u69d8<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u5e8f\u7ae0<\/p><\/td>

\u30d3\u30bf\u30df\u30f3 B12 (\u30b3\u30d0\u30e9\u30df\u30f3) \u306f\u3001\u7d30\u83cc\u3084\u53e4\u7d30\u83cc\u306b\u3088\u3063\u3066\u751f\u6210\u3055\u308c\u308b\u6c34\u6eb6\u6027\u30d3\u30bf\u30df\u30f3\u3067\u3059\u3002\u305d\u308c\u306f\u3001DNA\u5408\u6210\u3001\u8102\u80aa\u9178\u4ee3\u8b1d\u3001\u304a\u3088\u3073\u30a2\u30df\u30ce\u9178\u4ee3\u8b1d\u306e\u305f\u3081\u306b\u4ee3\u8b1d\u3059\u308b\u3059\u3079\u3066\u306e\u30d2\u30c8\u7d30\u80de\u306b\u3088\u3063\u3066\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u30d3\u30bf\u30df\u30f3\u306f\u3001\u4e2d\u5fc3\u306b\u30b3\u30d0\u30eb\u30c8\u30a4\u30aa\u30f3\u3092\u6301\u3064\u4fee\u98fe\u3055\u308c\u305f\u30b3\u30ea\u30f3\u74b0\u3067\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30d3\u30bf\u30df\u30f3B12\u304c\u6b20\u4e4f\u3059\u308b\u3068\u3001\u75b2\u52b4\u3001\u8ca7\u8840\u3001\u795e\u7d4c\u969c\u5bb3\u3092\u5f15\u304d\u8d77\u3053\u3057\u307e\u3059\u3002<\/p><\/td><\/tr>

\u76ee\u6a19<\/p><\/td>

\u30d3\u30bf\u30df\u30f3B12\uff08\u30b7\u30a2\u30ce\u30b3\u30d0\u30e9\u30df\u30f3\uff09<\/p><\/td><\/tr>

\u53cd\u5fdc\u6027<\/p><\/td>

\u4e00\u822c\uff08\u5168\u7a2e\uff09<\/p><\/td><\/tr>

\u30c6\u30b9\u30c8\u6e08\u307f\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/p><\/td>

\u30a8\u30ea\u30b5<\/p><\/td><\/tr>

\u63a8\u5968\u5e0c\u91c8\u7387<\/p><\/td>

\u6700\u9069\u306a\u5e0c\u91c8\/\u6fc3\u5ea6\u306f\u30a8\u30f3\u30c9\u30e6\u30fc\u30b6\u30fc\u304c\u6c7a\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p><\/td><\/tr>

\u4fdd\u7ba1\u6240<\/p><\/td>

4\u2103\u3067\u51fa\u8377\u3002\u30ad\u30c3\u30c8\u3092\u53d7\u3051\u53d6\u3063\u305f\u3089\u3001\u30ad\u30c3\u30c8\u306e\u30de\u30cb\u30e5\u30a2\u30eb\u306e\u4fdd\u7ba1\u6307\u793a\u306b\u5f93\u3063\u3066\u4fdd\u7ba1\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p><\/td><\/tr>

\u6709\u52b9<\/p><\/td>

\u3053\u306e\u30ad\u30c3\u30c8\u306e\u6709\u52b9\u671f\u9650\u306f 6 \u304b\u6708\u3067\u3059\u3002<\/p><\/td><\/tr>

\u5b89\u5b9a<\/p><\/td>

\u30ad\u30c3\u30c8\u306e\u5b89\u5b9a\u6027\u306f\u3001\u6d3b\u6027\u640d\u5931\u306e\u901f\u5ea6\u306b\u3088\u3063\u3066\u6c7a\u307e\u308a\u307e\u3059\u3002\u9069\u5207\u306a\u4fdd\u7ba1\u6761\u4ef6\u4e0b\u3067\u306f\u3001\u6709\u52b9\u671f\u9650\u5185\u306e\u640d\u5931\u7387\u306f 5% \u672a\u6e80\u3067\u3059\u3002\u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u306e\u5909\u52d5\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u306b\u306f\u3001\u64cd\u4f5c\u624b\u9806\u3068\u5b9f\u9a13\u5ba4\u306e\u6761\u4ef6\u3092\u53b3\u5bc6\u306b\u7ba1\u7406\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u30a2\u30c3\u30bb\u30a4\u5168\u4f53\u3092\u540c\u3058\u30e6\u30fc\u30b6\u30fc\u304c\u5b9f\u884c\u3059\u308b\u3053\u3068\u3092\u5f37\u304f\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p><\/td><\/tr>

\u8a66\u9a13\u7bc4\u56f2<\/p><\/td>

0.156 ng\/ml \u2013 10 ng\/ml<\/p><\/td><\/tr>

\u6a19\u6e96\u5f62\u5f0f<\/p><\/td>

\u51cd\u7d50\u4e7e\u71e5<\/p><\/td><\/tr>

\u691c\u51fa\u65b9\u6cd5<\/p><\/td>

\u6bd4\u8272\u5206\u6790<\/p><\/td><\/tr>

\u30a2\u30c3\u30bb\u30a4\u30c7\u30fc\u30bf<\/p><\/td>

\u5b9a\u91cf\u7684<\/p><\/td><\/tr>

\u30b5\u30f3\u30d7\u30eb\u306e\u7a2e\u985e<\/p><\/td>

\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d44\u7e54\u30db\u30e2\u30b8\u30cd\u30fc\u30c8\u304a\u3088\u3073\u305d\u306e\u4ed6\u306e\u4f53\u6db2\u3002<\/p><\/td><\/tr>

\u5206\u6790\u539f\u7406<\/p><\/td>

\u3053\u306e\u30ad\u30c3\u30c8\u306f\u3001\u30b5\u30f3\u30c9\u30a4\u30c3\u30c1\u9175\u7d20\u7d50\u5408\u514d\u75ab\u5438\u7740\u30a2\u30c3\u30bb\u30a4\u6280\u8853\u306b\u57fa\u3065\u3044\u3066\u3044\u307e\u3059\u3002\u6297\u4f53\u306f96\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u306b\u30d7\u30ec\u30b3\u30fc\u30c8\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u6a19\u6e96\u7269\u8cea\u3001\u8a66\u9a13\u30b5\u30f3\u30d7\u30eb\u3001\u304a\u3088\u3073\u30d3\u30aa\u30c1\u30f3\u7d50\u5408\u8a66\u85ac\u304c\u30a6\u30a7\u30eb\u306b\u6dfb\u52a0\u3055\u308c\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3055\u308c\u307e\u3059\u3002\u6b21\u306b\u3001HRP \u7d50\u5408\u8a66\u85ac\u3092\u6dfb\u52a0\u3057\u3001\u30d7\u30ec\u30fc\u30c8\u5168\u4f53\u3092\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002\u672a\u7d50\u5408\u306e\u30b3\u30f3\u30b8\u30e5\u30b2\u30fc\u30c8\u306f\u3001\u5404\u6bb5\u968e\u3067\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u4f7f\u7528\u3057\u3066\u9664\u53bb\u3055\u308c\u307e\u3059\u3002 TMB \u57fa\u8cea\u306f\u3001HRP \u9175\u7d20\u53cd\u5fdc\u3092\u5b9a\u91cf\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002 TMB \u57fa\u8cea\u3092\u6dfb\u52a0\u3059\u308b\u3068\u3001\u5341\u5206\u306a\u30d3\u30bf\u30df\u30f3 B12 \u3092\u542b\u3080\u30a6\u30a7\u30eb\u306e\u307f\u304c\u9752\u8272\u306e\u751f\u6210\u7269\u3092\u751f\u6210\u3057\u3001\u9178\u6027\u505c\u6b62\u6db2\u3092\u6dfb\u52a0\u3059\u308b\u3068\u9ec4\u8272\u306b\u5909\u308f\u308a\u307e\u3059\u3002\u9ec4\u8272\u306e\u5f37\u5ea6\u306f\u3001\u30d7\u30ec\u30fc\u30c8\u306b\u7d50\u5408\u3057\u305f\u30d3\u30bf\u30df\u30f3 B12 \u306e\u91cf\u306b\u6bd4\u4f8b\u3057\u307e\u3059\u3002\u5149\u5b66\u5bc6\u5ea6 (OD) \u306f\u3001\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8 \u30ea\u30fc\u30c0\u30fc\u3067 450 nm \u3067\u5206\u5149\u6e2c\u5149\u7684\u306b\u6e2c\u5b9a\u3055\u308c\u3001\u305d\u3053\u304b\u3089\u30d3\u30bf\u30df\u30f3 B12 \u306e\u6fc3\u5ea6\u3092\u8a08\u7b97\u3067\u304d\u307e\u3059\u3002<\/p><\/td><\/tr>

\u30ad\u30c3\u30c8\u306e\u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8<\/p><\/td>

\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u30ad\u30c3\u30c8\u306e\u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8\u306f\u53c2\u8003\u7528\u3067\u3059\u3002\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u3068\u306f\u82e5\u5e72\u7570\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u88fd\u54c1\u306f\u3001\u88fd\u54c1\u306b\u540c\u68b1\u304a\u3088\u3073\u7d0d\u54c1\u3055\u308c\u308b\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u306e\u8a18\u8f09\u306b\u5f93\u3063\u3066\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>

\u30fb\u30d7\u30ec\u30b3\u30fc\u30c896\u30a6\u30a7\u30eb\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8<\/p>

\u30fb \u6a19\u6e96<\/p>

\u30fb\u6a19\u6e96\u5e0c\u91c8\u7de9\u885d\u6db2<\/p>

\u30fb\u30a6\u30a9\u30c3\u30b7\u30e5\u30d0\u30c3\u30d5\u30a1\u30fc<\/p>

\u30fb\u691c\u51fa\u8a66\u85acA<\/p>

\u30fb\u691c\u51fa\u8a66\u85acB<\/p>

\u30fb\u5e0c\u91c8\u5264A<\/p>

\u30fb\u5e0c\u91c8\u5264B<\/p>

\u30fbTMB\u57fa\u677f<\/p>

\u30fb\u30b9\u30c8\u30c3\u30d7\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/p>

\u30fb\u30d7\u30ec\u30fc\u30c8\u30b7\u30fc\u30e9\u30fc<\/p><\/td><\/tr>

\u5fc5\u8981\u306a\u8cc7\u6599\u304c\u63d0\u4f9b\u3055\u308c\u3066\u3044\u306a\u3044<\/p><\/td>

\u30fb37\u2103\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc<\/p>

\u00b7 \u30de\u30eb\u30c1\u304a\u3088\u3073\u30b7\u30f3\u30b0\u30eb\u30c1\u30e3\u30f3\u30cd\u30eb\u30d4\u30da\u30c3\u30c8\u304a\u3088\u3073\u6ec5\u83cc\u30d4\u30da\u30c3\u30c8\u30c1\u30c3\u30d7<\/p>

\u30fb\u5674\u51fa\u30dc\u30c8\u30eb\u307e\u305f\u306f\u81ea\u52d5\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u6d17\u6d44\u6a5f<\/p>

\u30fb1.5ml\u30c1\u30e5\u30fc\u30d6<\/p>

\u30fb \u84b8\u7559\u6c34<\/p>

\u30fb\u5438\u53ce\u6ffe\u7d19<\/p>

\u30fb100ml\u30681\u30ea\u30c3\u30c8\u30eb\u306e\u30e1\u30b9\u30b7\u30ea\u30f3\u30c0\u30fc<\/p>

\u30fb\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\uff08\u6ce2\u9577\uff1a450nm\uff09<\/p>

\u30fbELISA\u30b7\u30a7\u30fc\u30ab\u30fc<\/p><\/td><\/tr>

\u8a66\u85ac\u306e\u6e96\u5099<\/p><\/td>

\u3053\u306e\u624b\u9806\u306f\u53c2\u8003\u306e\u305f\u3081\u306b\u306e\u307f\u63d0\u4f9b\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u3068\u306f\u82e5\u5e72\u7570\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u88fd\u54c1\u306f\u3001\u88fd\u54c1\u306b\u540c\u68b1\u304a\u3088\u3073\u7d0d\u54c1\u3055\u308c\u308b\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u306e\u8a18\u8f09\u306b\u5f93\u3063\u3066\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>

\u30fb1) \u6a19\u6e96\u6db2\uff1a\u63a8\u5968\u91cf\u306e\u6a19\u6e96\u5e0c\u91c8\u7de9\u885d\u6db2\u3092\u7528\u3044\u3066\u6a19\u6e96\u6db2\u3092\u8abf\u88fd\u3057\u3001\u6a19\u6e96\u6db2\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002\u6b21\u306b\u3001\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306e\u6307\u793a\u306b\u5f93\u3063\u3066\u3001\u6a19\u6e96\u5e0c\u91c8\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u4f7f\u7528\u3057\u3066\u6a19\u6e96\u6eb6\u6db2\u306e\u6bb5\u968e\u5e0c\u91c8\u3092\u5b9f\u884c\u3057\u307e\u3059\u3002<\/p>

\u00b7 2) \u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc: \u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306e\u6307\u793a\u306b\u5f93\u3063\u3066\u3001\u6fc3\u7e2e\u3057\u305f\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u84b8\u7559\u6c34\u3067\u5e0c\u91c8\u3057\u307e\u3059\u3002<\/p>

\u00b7 3) \u691c\u51fa\u8a66\u85ac\u306e\u8abf\u88fd: \u5fc5\u8981\u306a\u4f5c\u696d\u6eb6\u6db2\u306e\u7dcf\u91cf\u3092\u8a08\u7b97\u3057\u307e\u3059\u3002\u691c\u51fa\u8a66\u85ac A \u3068\u691c\u51fa\u8a66\u85ac B \u3092\u305d\u308c\u305e\u308c\u5e0c\u91c8\u6db2 A \u3068\u5e0c\u91c8\u6db2 B \u3067 1:100 \u306b\u5e0c\u91c8\u3057\u307e\u3059\u3002<\/p><\/td><\/tr>

\u30a2\u30c3\u30bb\u30a4\u624b\u9806<\/p><\/td>

\u3053\u306e\u624b\u9806\u306f\u53c2\u8003\u306e\u305f\u3081\u306b\u306e\u307f\u63d0\u4f9b\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u3068\u306f\u82e5\u5e72\u7570\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u88fd\u54c1\u306f\u3001\u88fd\u54c1\u306b\u540c\u68b1\u304a\u3088\u3073\u7d0d\u54c1\u3055\u308c\u308b\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u306e\u8a18\u8f09\u306b\u5f93\u3063\u3066\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>

\u00b7 1) \u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3001\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30a6\u30a7\u30eb\u3092\u8a2d\u5b9a\u3057\u307e\u3059\u3002<\/p>

\u00b7 2) \u5e0c\u91c8\u3057\u305f\u6a19\u6e96\u6db2 100 \u03bcl \u3092\u6a19\u6e96\u30a6\u30a7\u30eb\u306b\u5206\u53d6\u3057\u307e\u3059\u3002<\/p>

\u00b7 3) \u6a19\u6e96\u5e0c\u91c8\u30d0\u30c3\u30d5\u30a1\u30fc 100 \u03bcl \u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb (\u30bc\u30ed) \u30a6\u30a7\u30eb\u306b\u5206\u6ce8\u3057\u307e\u3059\u3002<\/p>

\u30fb 4) \u5e0c\u91c8\u30b5\u30f3\u30d7\u30eb 100 \u03bcl \u3092\u30b5\u30f3\u30d7\u30eb\u30a6\u30a7\u30eb\u306b\u5206\u53d6\u3057\u307e\u3059\u3002 37\u2103\u30671\u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>

\u30fb5) \u691c\u51fa\u8a66\u85ac A 100 \u03bcl \u3092\u5404\u30a6\u30a7\u30eb\u306b\u5206\u6ce8\u3057\u307e\u3059\u3002 37\u2103\u30671\u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>

\u30fb6) 3\u56de\u6d17\u3044\u307e\u3059\u3002<\/p>

\u30fb 7) \u691c\u51fa\u8a66\u85ac B 100 \u03bcl \u3092\u5404\u30a6\u30a7\u30eb\u306b\u5206\u6ce8\u3057\u307e\u3059\u3002 37\u2103\u306730\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>

\u30fb8) 5\u56de\u6d17\u3044\u307e\u3059\u3002<\/p>

\u30fb\uff19\uff09\uff34\uff2d\uff22\u57fa\u8cea\uff19\uff10\u03bc\uff4c\u3092\u5404\u30a6\u30a7\u30eb\u306b\u5206\u53d6\u3059\u308b\u3002 37\u2103\u306710\u301c20\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>

\u00b7 10) \u505c\u6b62\u6db2\u3092 50 \u03bcl \u306b\u5206\u6ce8\u3057\u307e\u3059\u3002<\/p>

\u30fb\uff11\uff11\uff09\uff14\uff15\uff10\uff4e\uff4d\u3067\uff2f\uff24\u3092\u6e2c\u5b9a\u3059\u308b\u3002<\/p><\/td><\/tr>

\u30d7\u30ed\u30c8\u30b3\u30eb<\/p><\/td>

\u3053\u306e\u624b\u9806\u306f\u53c2\u8003\u306e\u305f\u3081\u306b\u306e\u307f\u63d0\u4f9b\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u3068\u306f\u82e5\u5e72\u7570\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u88fd\u54c1\u306f\u3001\u88fd\u54c1\u306b\u540c\u68b1\u304a\u3088\u3073\u7d0d\u54c1\u3055\u308c\u308b\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u306e\u8a18\u8f09\u306b\u5f93\u3063\u3066\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>

\u00b7 \u4f7f\u7528\u524d\u306b\u30ad\u30c3\u30c8\u306e\u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8\u3068\u30b5\u30f3\u30d7\u30eb\u3092\u5ba4\u6e29 (18 \uff5e 25 \u00b0C) \u306b\u5e73\u8861\u5316\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30c6\u30b9\u30c8\u3054\u3068\u306b\u6a19\u6e96\u66f2\u7dda\u3092\u30d7\u30ed\u30c3\u30c8\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p>

\u30fb1. \u30d7\u30ec\u30b3\u30fc\u30c8\u30d7\u30ec\u30fc\u30c8\u4e0a\u306b\u6a19\u6e96\u30a6\u30a7\u30eb\u3001\u8a66\u9a13\u30b5\u30f3\u30d7\u30eb\u30a6\u30a7\u30eb\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08\u30bc\u30ed\uff09\u30a6\u30a7\u30eb\u3092\u305d\u308c\u305e\u308c\u30bb\u30c3\u30c8\u3057\u3001\u305d\u306e\u4f4d\u7f6e\u3092\u8a18\u9332\u3057\u307e\u3059\u3002\u5404\u6a19\u6e96\u304a\u3088\u3073\u30b5\u30f3\u30d7\u30eb\u3092\u5c11\u306a\u304f\u3068\u3082 2 \u56de\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p>

\u00b7 2. \u5404\u6a19\u6e96\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3001\u304a\u3088\u3073\u30b5\u30f3\u30d7\u30eb\u306e 100 \u00b5L \u3092\u9069\u5207\u306a\u30a6\u30a7\u30eb\u306b\u52a0\u3048\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u3092\u30ab\u30d0\u30fc\u3067\u5bc6\u9589\u3057\u300137\u2103\u3067 1 \u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>

\u30fb3.\u30ab\u30d0\u30fc\u3092\u5916\u3057\u3001\u6db2\u3092\u6368\u3066\u307e\u3059\u3002<\/p>

\u00b7 4. 100 \u03bcl \u306e\u691c\u51fa\u8a66\u85ac A \u4f7f\u7528\u6eb6\u6db2\u3092\u5404\u30a6\u30a7\u30eb\u306b\u52a0\u3048\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u3092\u30ab\u30d0\u30fc\u3067\u5bc6\u9589\u3057\u300137\u2103\u3067 1 \u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>

\u30fb5.\u30ab\u30d0\u30fc\u3092\u5916\u3057\u3001\u6eb6\u6db2\u3092\u6368\u3066\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u3092 1X \u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc\u3067 3 \u56de\u6d17\u6d44\u3057\u307e\u3059\u3002<\/p>

\u00b7 6. 100 \u03bcL \u306e\u691c\u51fa\u8a66\u85ac B \u4f5c\u696d\u6eb6\u6db2\u3092\u5404\u30a6\u30a7\u30eb\u306b\u52a0\u3048\u3001\u5bc6\u9589\u3057\u300137\u00b0C \u3067 30 \u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>

\u30fb 7. \u6eb6\u6db2\u3092\u6368\u3066\u3001\u524d\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u8aac\u660e\u3057\u305f\u3088\u3046\u306b\u6d17\u6d44\u7de9\u885d\u6db2\u3067\u30d7\u30ec\u30fc\u30c8\u3092 5 \u56de\u6d17\u6d44\u3057\u307e\u3059\u3002<\/p>

\u30fb 8. 90 \u03bcl \u306e TMB \u57fa\u8cea\u3092\u5404\u30a6\u30a7\u30eb\u306b\u5206\u6ce8\u3057\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u3092\u30ab\u30d0\u30fc\u3067\u5bc6\u9589\u3057\u300137\u2103\u306710\u301c20\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002\u5149\u3078\u306e\u66dd\u9732\u3092\u907f\u3051\u3066\u304f\u3060\u3055\u3044\u3002\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u6642\u9593\u306f\u53c2\u8003\u7528\u3067\u3059\u3002\u6700\u9069\u306a\u6642\u9593\u306f\u30a8\u30f3\u30c9\u30e6\u30fc\u30b6\u30fc\u304c\u6c7a\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002 30 \u5206\u3092\u8d85\u3048\u306a\u3044\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>

\u00b7 9. \u5404\u30a6\u30a7\u30eb\u306b 50 \u03bcL \u306e\u505c\u6b62\u6db2\u3092\u52a0\u3048\u307e\u3059\u3002 450nm\u3067\u3059\u3050\u306b\u8aad\u307f\u53d6\u308a\u307e\u3059\u3002<\/p><\/td><\/tr>

\u7d50\u679c\u306e\u8a08\u7b97<\/p><\/td>

\u8a08\u7b97\u3067\u306f\u3001\u5404\u53c2\u7167\u6a19\u6e96\u304a\u3088\u3073\u5404\u30b5\u30f3\u30d7\u30eb\u306e\u91cd\u8907\u3057\u305f OD450 \u6e2c\u5b9a\u5024\u3092\u5e73\u5747\u3057\u3001\u5e73\u5747\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb (\u30bc\u30ed) OD450 \u6e2c\u5b9a\u5024\u3092\u6e1b\u7b97\u3057\u307e\u3059\u3002\u6a19\u6e96\u66f2\u7dda\u306f\u3001\u5404\u53c2\u7167\u6a19\u6e96\u6eb6\u6db2\u306e\u76f8\u5bfe OD450 (Y) \u5bfe\u5404\u6a19\u6e96\u6eb6\u6db2\u306e\u305d\u308c\u305e\u308c\u306e\u6fc3\u5ea6 (X) \u3068\u3057\u3066\u30d7\u30ed\u30c3\u30c8\u3067\u304d\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u30d3\u30bf\u30df\u30f3 B12 \u6fc3\u5ea6\u306f\u6a19\u6e96\u66f2\u7dda\u304b\u3089\u88dc\u9593\u3067\u304d\u307e\u3059\u3002<\/p><\/td><\/tr>

\u53ef\u7528\u6027<\/p><\/td>

5\uff5e12\u55b6\u696d\u65e5\u4ee5\u5185\u306b\u767a\u9001\u3055\u308c\u307e\u3059\u3002<\/p><\/td><\/tr>

\u30ce\u30fc\u30c8<\/p><\/td>

\u3053\u306e\u88fd\u54c1\u306f\u7814\u7a76\u5c02\u7528\u3067\u3059\u3002<\/p>

\u7bc4\u56f2\u3068\u611f\u5ea6\u306f\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u6700\u65b0\u306e\u88fd\u54c1\u60c5\u5831\u306b\u3064\u3044\u3066\u306f\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002\u6b63\u78ba\u306a\u7d50\u679c\u3092\u5f97\u308b\u306b\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u3092\u30ad\u30c3\u30c8\u306e\u4e2d\u9593\u6fc3\u5ea6\u306b\u5e0c\u91c8\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u7279\u5b9a\u306e\u7bc4\u56f2\u3092\u3054\u5e0c\u671b\u306e\u5834\u5408\u306f\u3001\u4e8b\u524d\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u3044\u305f\u3060\u304f\u304b\u3001\u3054\u6ce8\u6587\u306e\u30b3\u30e1\u30f3\u30c8\u306b\u30ea\u30af\u30a8\u30b9\u30c8\u3092\u8a18\u8f09\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>

\u5f53\u793e\u306e ELISA \u30ad\u30c3\u30c8\u304a\u3088\u3073 CLIA \u30ad\u30c3\u30c8\u306f\u3001\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u306f\u306a\u304f\u30cd\u30a4\u30c6\u30a3\u30d6\u30b5\u30f3\u30d7\u30eb\u306e\u691c\u51fa\u7528\u306b\u6700\u9069\u5316\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u5929\u7136\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306f\u7570\u306a\u308b\u914d\u5217\u3084\u4e09\u6b21\u69cb\u9020\u3092\u6301\u3063\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001\u5f53\u793e\u3067\u306f\u305d\u306e\u691c\u51fa\u3092\u4fdd\u8a3c\u3067\u304d\u307e\u305b\u3093\u3002<\/p><\/td><\/tr>

\u3067\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u3055\u308c\u305f\u30d7\u30ec\u30fc\u30c8<\/p><\/td>

\u6297\u539f<\/p><\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

\u30d3\u30bf\u30df\u30f3 B12 ELISA \u30ad\u30c3\u30c8 \u30d3\u30bf\u30df\u30f3 B12 (\u30b7\u30a2\u30ce\u30b3\u30d0\u30e9\u30df\u30f3) ELISA \u30ad\u30c3\u30c8\u306f\u3001\u2026<\/p>\n

\u30d3\u30bf\u30df\u30f3B12 ELISA\u30ad\u30c3\u30c8<\/span> \u3082\u3063\u3068\u8a73\u3057\u304f\u77e5\u308b "<\/a><\/p>","protected":false},"author":1,"featured_media":3080,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[37,48],"tags":[24],"class_list":["post-2136","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-elisa","category-products","tag-dairy"],"_links":{"self":[{"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/posts\/2136","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/comments?post=2136"}],"version-history":[{"count":0,"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/posts\/2136\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/media\/3080"}],"wp:attachment":[{"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/media?parent=2136"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/categories?post=2136"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/ja\/wp-json\/wp\/v2\/tags?post=2136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}