{"id":2304,"date":"2023-04-19T07:01:53","date_gmt":"2023-04-19T07:01:53","guid":{"rendered":"https:\/\/www.vnyabiotech.com\/?p=2304"},"modified":"2023-04-26T02:58:38","modified_gmt":"2023-04-26T02:58:38","slug":"malachite-green-residue-detection-test-strip","status":"publish","type":"post","link":"https:\/\/www.vnyabiotech.com\/es\/malachite-green-residue-detection-test-strip\/","title":{"rendered":"Tira de prueba de detecci\u00f3n de residuos de verde malaquita"},"content":{"rendered":"
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Tira de prueba de detecci\u00f3n de residuos de verde malaquita<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Residuos de verde de malaquita (MG), un f\u00e1rmaco veterinario utilizado ilegalmente para el tratamiento de peces de acuicultura. La detecci\u00f3n de residuos de aditivos ilegales, como el verde de malaquita en los mariscos y el agua, es motivo de gran preocupaci\u00f3n para la acuicultura debido a los efectos adversos y de interferencia potenciales de los residuos de aditivos ilegales que se encuentran con frecuencia en los mariscos y el agua.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u00c1mbito de aplicaci\u00f3n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u3010Principios de uso\u3011<\/p>

El kit de prueba ELISA verde de malaquita es un inmunoensayo marcado con enzima competitivo indirecto. El ant\u00edgeno verde de malaquita se precubre en los pocillos. El ant\u00edgeno recubierto compite con el anticuerpo verde de malaquita (soluci\u00f3n de anticuerpos) con el verde de malaquita en la muestra, el anticuerpo anti-verde de malaquita se une al conjugado de enzima verde de malaquita-HRP. A continuaci\u00f3n, pipetee las soluciones de sustrato en los pocillos para convertir el color.<\/p>

\u00a0<\/p>

El color de las muestras desconocidas se compara con el color de los est\u00e1ndares y se obtiene la concentraci\u00f3n de verde malaquita de las muestras.<\/p>

\u3010Reactividad cruzada\u3011<\/p>

Verde malaquita\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026..\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026100%<\/p>

Verde leuco-malaquita (oxidado)\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026.\u2026\u2026\u2026\u2026\u2026\u2026100%<\/p>

Cristal violeta\u2026\u2026\u2026\u2026\u2026\u2026.\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026150%<\/p>

Leuco-cristal violeta\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026\u2026.150%<\/p>

\u3010Materiales necesarios pero no proporcionados\u3011<\/p>

Dispositivos:<\/p>

\u2014- Lector de microplacas (filtro de 450nm\/630nm)<\/p>

\u2014- Homogeneizador<\/p>

\u2014- V\u00f3rtice<\/p>

\u2014- Centr\u00edfuga<\/p>

\u2014- Incubadora de temperatura constante<\/p>

\u2014- Equilibrio (sensibilidad: 0,01g)<\/p>

\u2014- Pipeta graduada: 10mL<\/p>

\u2014- Concentrador de muestras Termovap<\/p>

\u2014- Jeringa de bulbo de goma<\/p>

\u2014- Tubo de centr\u00edfuga: 5mL\/7mL, 50mL<\/p>

\u2014- Micropipeta: Pipeta monocanal (10-100 \u00b5L, 100-1000 \u00b5L)<\/p>

Pipeta multicanal (30-300 \u00b5L)<\/p>

Reactivo:<\/p>

-- Agua desionizada<\/p>

\u2014- Acetonitrilo (AR)<\/p>

\u2014- \u00c1cido sulf\u00farico concentrado 18M (H2SO4)<\/p>

\u2014- Hidr\u00f3xido de sodio (NaOH) (AR)<\/p>

\u3010Materiales y reactivos proporcionados\u3011<\/p>

Componente 96 pozos<\/p>

Placa recubierta de ant\u00edgeno 96T<\/p>

5 viales de 1 ml est\u00e1ndar cada uno<\/p>

Est\u00e1ndar de alta concentraci\u00f3n (100ppb) 1mL<\/p>

Soluci\u00f3n de anticuerpos 6mL<\/p>

Conjugado enzim\u00e1tico 12mL<\/p>

Sustrato A 6mL<\/p>

Sustrato B 6mL<\/p>

Tamp\u00f3n de lavado concentrado (10\u00d7) 40 ml<\/p>

Tamp\u00f3n de diluci\u00f3n de muestra \u00d7 2 viales 45 ml\/vial<\/p>

Extractor de muestra A \u00d72 bolsas 90 g\/bolsa<\/p>

Extractor de muestra B \u00d72 bolsas 90 g\/bolsa<\/p>

Oxidante 6mL<\/p>

Prospecto 1<\/p>

Informe de prueba 1<\/p>

Hoja de cubierta de placa 2<\/p>

Nota:<\/p>

Las concentraciones de los est\u00e1ndares son 0ppb, 0.03ppb, 0.09ppb, 0.27ppb y 0.81ppb.<\/p>

Extractor de muestra A y B: despu\u00e9s de cada prueba, selle la bolsa con cierre herm\u00e9tico y col\u00f3quela en la bolsa de papel de aluminio con desecante para volver a sellarla.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Tira de prueba de detecci\u00f3n de residuos de verde de malaquita Los residuos de verde de malaquita (MG), un medicamento veterinario ilegalmente \u2026<\/p>\n

Tira de prueba de detecci\u00f3n de residuos de verde malaquita<\/span> Aprende m\u00e1s "<\/a><\/p>","protected":false},"author":1,"featured_media":3063,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[48,38],"tags":[44],"class_list":["post-2304","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-products","category-rapid-test-kid","tag-seafood"],"_links":{"self":[{"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/posts\/2304","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/comments?post=2304"}],"version-history":[{"count":0,"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/posts\/2304\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/media\/3063"}],"wp:attachment":[{"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/media?parent=2304"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/categories?post=2304"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vnyabiotech.com\/es\/wp-json\/wp\/v2\/tags?post=2304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}