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The commonly used methods for detecting mycotoxin can generally be divided into two categories. One is the rapid detection method, including enzyme-linked immunosorbent assay, immunochromatography (colloidal gold\/fluorescence), etc; One type is instrumental methods, including high-performance liquid chromatography, high-performance liquid chromatography tandem mass spectrometry, etc; The rapid detection method has the advantages of simple operation, low personnel requirements, easy portability, and low cost. However, there are also significant issues with the accuracy and stability of the detection results. Therefore, it is generally only used as a preliminary screening test for samples and cannot be used as an accurate quantitative method. The instrument method is generally a national or industry standard testing method, which has high accuracy and precision in its testing results. Therefore, it is generally used in some large group central laboratories, testing centers, government food testing units, customs, grain and oil quality inspection centers, third-party testing laboratories, etc. However, due to the cumbersome operation, low purification efficiency, and low degree of automation of the sample pretreatment technology of the existing instrument method, it seriously affects the accuracy and work efficiency of the testing results. Therefore, it is particularly important to establish a simple, efficient, and automated sample pretreatment technology throughout the entire sample analysis process, which is the fundamental step for achieving efficient and accurate detection of mycotoxin.<\/p>\n
Immunomagnetic beads (IMB) technology is a newly developed separation technique that combines highly specific immunological reactions of antigen antibody reactions with the unique advantages of solidifying reagents. The principle of its separation technology is that the surface modification of nano magnetic beads containing Fe3O4 with different functional groups binds to antibodies to form immune magnetic beads, which specifically bind to the corresponding antigens in the sample, forming antigen antibody magnetic bead complexes. The force acting on the enrichment and purification system is the external magnetic field. Immune magnetic bead particles can undergo directional mechanical movement in the magnetic field and quickly aggregate under the action of the magnetic field. Due to the good dispersibility of magnetic microspheres, antigen antibody separation can be achieved under specific elution conditions, allowing for rapid separation, purification, and enrichment of target components using magnets after the reaction is complete. In recent years, many studies have shown that using immunomagnetic bead purification for sample pretreatment can simplify the pretreatment process of instrument based detection of mycotoxin, improve the automation level and detection efficiency of detection, and meet the rapid and accurate quantitative detection of large quantities of samples.<\/p>\n
The JLZC\/VNYA mycotoxin fully automatic purifier is a mycotoxin purification device developed for large-scale sample pretreatment applications, using fully automatic immunomagnetic bead purification technology. After extracting mycotoxin from the sample, the mycotoxins in the extraction solution are specifically adsorbed onto antibodies on the surface of magnetic beads. The entire process of fully automatic high-throughput magnetic suction, transfer, washing, and elution is completed through a built-in magnetic rod, and different terminal detection instruments (such as high-performance liquid chromatography, ultra high performance liquid chromatography, liquid chromatography-mass spectrometry, etc.) can be used to achieve rapid, high-throughput, and high-precision analysis and testing of mycotoxin in the sample.<\/p>\n
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Compared with the affinity column purification method, the JLZC\/VNYA mycotoxin fully automatic purifier can significantly simplify the sample pretreatment process, improve the automation level and detection efficiency of detection, meet the rapid and accurate quantitative detection of large quantities of samples, and also avoid human operation errors, ensuring the accuracy of operation.<\/p>\n
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The mycotoxin immunomagnetic bead kit is a specialized kit developed by VNYA and JLZC for rapid and high-throughput sample purification in mycotoxin detection. After extracting mycotoxin from the sample, the mycotoxin in the extraction solution are specifically adsorbed onto antibodies on the surface of magnetic beads. Combined with a fully automatic mycotoxin purifier, the entire process of impurity washing and target purification and elution can be completed.<\/p>\n
The mycotoxin immunomagnetic bead kit covers common toxin items for daily testing, including aflatoxin B1, vomitoxin, zearalenone, ochratoxin A, fumarate toxin, and T2 toxin etc.<\/p>\n
The mycotoxin immunomagnetic bead kit has the following characteristics:<\/p>\n
a) Easy to use: tear and use immediately, add extraction solution directly, no need to prepare other solutions.<\/p>\n
b) Automatic and efficient: Combined with a fully automatic mycotoxin purifier, batch processing is possible without the need for manual operation.<\/p>\n
c) Reliable results: no risk of blockage, high method recovery rate, and good repeatability of results.<\/p>\n
d) Precise purification: Customize purification programs for different substrates to achieve better purification effects.<\/p>\n
The fully automatic purification instrument for mycotoxin, combined with the mycotoxin immunomagnetic bead kit, is suitable for rapid and high-throughput purification of samples during the detection of mycotoxin in grains, oils, feed, Chinese medicinal materials, seasonings, and various types of samples. It has the characteristics of convenient operation, high efficiency and speed, stable operation, safety and reliability.<\/p>\n
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The commonly used methods for detecting mycotoxin can generally be divided into two categories. One …<\/p>\n