β-Lactams & Sulfonamides & TetracyclinesTriple Test strip
This kit is based on the specific reaction of antibody-antigen and immunochromatography. β-lactams, sulfonamides and tetracyclines antibiotics in the sample compete for the antibody with the antigen coated on the membrane of the test strip. Then after a color reaction, the result can be observed.
Scope of application
This kit is used for rapid qualitative analysis of β-lactams , sulfonamides and tetracyclines in raw milk sample.
Detection limit
β-lactams |
MRL(μg/L) |
LOD(μg/L) |
Tetracyclines |
MRL(μg/L) |
LOD(μg/L) |
penicillin G |
4 |
2-2.5 |
tetracycline | 100 | 25-30 |
ampicillin |
4 |
3-4 |
oxytetracycline | 100 | 25-30 |
amoxicillin |
4 |
3-4 |
doxycycline | 100 | 30-35 |
oxacillin |
30 |
5-6 |
chlortetracycline | 100 | 30-35 |
nafcillin |
30 |
14-16 |
Sulfonamides |
MRL(μg/L) |
LOD(μg/L) |
dicloxacillin |
30 |
4-5 |
sulfamethazine |
100 |
2-2.5 |
Cephalosporins |
MRL(μg/L) |
LOD(μg/L) |
sulfadimethoxine | 100 |
1-1.2 |
cefquinome |
20 |
10-12 |
sulfaquinoxaline | 100 | 0.8-1 |
cefoperazone |
50 |
4-5 |
sulfadiazine | 100 | 0.4-0.5 |
ceftiofur |
100 |
100 |
sulfapyridine | 100 | 18-20 |
Cefalonium |
20 |
3-4 |
sulfamonomethoxine | 100 | 0.3-0.4 |
cephacetrile |
125 |
35-40 |
sulfachlorpyridazine | 100 | 1.5-2 |
|
|
|
sulfamethoxypyridazine | 100 | 8-9 |
|
|
|
sulfamerazine | 100 | 1-1.5 |
|
|
|
sulfathiazole | 100 | 3-4 |
Product composition
l 8-well substrate and 8 test strip in one bottle. 12 bottles / kit. |
l Kit insert |
l microwell holder, 1pcs |
l Plastic pipette, 96pcs |
** Use one strip for one sample, the rest strips can be stored in the bottle for future use.
Self-prepared equipment and reagents
- stripReader
- Metalincubator
Test steps
（1）Read the instructions carefully before experiment. Bring the test kit and samples to room temperature. Milk samples should be fully liquid without any agglomeration or deposition.
（2）Take bottles needed from the kit package, take out required wells and strips, and make proper marks. Please use these test strip within 1h. Seal the cap of the bottles. The rest strip can be stored for future use.
（3）Place the mircrowells in the metal incubator, take 200ul of the test samples into the wells, then repeatedly absorb and drop for 5 times to mix the sample with the reagent in the wells completely. The mixture should be pink, and then start the timer.
（4）Incubate for 3min at ambient temperature (45℃); insert the test strip into the wells with the “Sample pad” end fully dipped in to the mixture.
（5）Incubate for 6min at ambient temperature (45℃) again. Take out the strip; judge the result according to Part 7.
Result determination
There are 4 lines in the strip, Control line, Beta-lactams Line, Sulfonamides Line and Tetracylcines Line, which are briefly used as “C”, “T1”,”T2” and “T3”. The test results will depend on the color of these lines. The following diagram describes the result identification.
Negative: Line C, Line T1 ,Line T2 and Line T3 are all red, color of Line T1 ,Line T2 and Line T3 are all darker than or similar to Line C, indicating the corresponding residue in sample is less than LOD of the kit.
Beta-lactams Positive: Line C is red, color of Line T1 is weaker than Line C, indicating the beta-lactams residue in sample is higher than LOD of the kit.
Sulfonamides Positive: Line C is red, color of Line T2 is weaker than Line C, indicating the sulfonamides residue in sample is higher than LOD of the kit.
Tetracyclines Positive: Line T is red, color of Line T3 is weaker than Line C, indicating the tetracyclines residue in
sample is higher than LOD of the kit.
Please notice: Control line is used as a quality indicator, which will always appear regardless of the B/T line. If Control line does NOT appear, this indicates that the result is invalid. Users please check the kit insert again and re-do the assay with new test strip.
Read the test strip with the instrument
